Coding

Part:BBa_K895002:Design

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-22)


Salty_VgrG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 474
    Illegal BglII site found at 1806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1048
    Illegal AgeI site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Salmonella vgrG gene did not require any modification to meet RFC[10] standard.


Source

Amplified by PCR from Salmonella LT2 genomic DNA.

References