Regulatory
Tat PROM

Part:BBa_K895000:Design

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-21)

Eco_Ptat2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 98
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part has an engineered BamHI (5'-GGATCC-3') restriction site at the extreme 3' end. For best results, open reading frames should be cloned into this BamHI site with their ATG initiation codons immediately following it. This will place the ATG at exactly the correct distance from the native tatA RBS for optimal translational efficiency.

Source

Amplified by PCR from BBa_K562000.

References

Jack RL, Sargent F, Berks BC, Sawers G, Palmer T. Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth. J Bacteriol. 2001 Mar;183(5):1801-4.