![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K890000:Design
Designed by: James Bevington Group: iGEM12_UGA-Georgia (2012-10-03)
methanococcus shuttle vector pAW42
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 234
Illegal XbaI site found at 243
Illegal SpeI site found at 4948
Illegal PstI site found at 772 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 4948
Illegal PstI site found at 772
Illegal NotI site found at 959 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 221
Illegal BamHI site found at 753 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 234
Illegal XbaI site found at 243
Illegal SpeI site found at 4948
Illegal PstI site found at 772 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 234
Illegal XbaI site found at 243
Illegal SpeI site found at 4948
Illegal PstI site found at 772
Illegal NgoMIV site found at 203
Illegal AgeI site found at 795
Illegal AgeI site found at 971 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3129
Illegal BsaI.rc site found at 1512
Illegal BsaI.rc site found at 1841
Illegal SapI site found at 4793
Design Notes
Clone DNA downstream from a strong promoter, Nde1 site.
Source
Walters, Alison D., Sarah Smith, and James Chong. "Shuttle Vector System for Methanococcus
maripaludis with Improved Transformation Efficiency." Applied and Environmental Microbiology
77.7 (2011): 2549-51. Print.