Composite

Part:BBa_K887071:Design

Designed by: Ting Chang   Group: iGEM12_NCTU_Formosa   (2012-09-09)


Plac(promoter)+aRBS(B0034)+zif268+alsS+a RBS(B0034)+PBSII+ilvC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
    Illegal AgeI site found at 2996
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2582
    Illegal BsaI.rc site found at 674
    Illegal BsaI.rc site found at 1268
    Illegal BsaI.rc site found at 3401


Design Notes

We had been afraid that it is too long to connect all of them become one part of our biobrick.Fortunately,we are successful for connecting all of them with no problems.


Source

We advance the 2011 igem NCTU_Formosa Team's idea to produce isobutanol more efficiently and successfully.


References