Device
Part:BBa_K883151:Design
Designed by: Jeroen Bosman Group: iGEM12_Wageningen_UR (2012-09-19)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Select the number of AA that gets deleted, to get the N terminal end of the CCMV more to the surface, to become available on the outside.
Source
an pET28 vector containing an inducable CCMV coat protein from Virology group WUR