Coding

Part:BBa_K874000:Design

Designed by: Ernst Bank   Group: iGEM12_Amsterdam   (2012-09-17)

M.ScaI Methyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No codon optimization performed.
Native sequence had to be silently mutated in order to remove the following [http://dspace.mit.edu/bitstream/handle/1721.1/45138/BBFRFC10.txt RFC-10] [1] forbidden/undesirable sites:

  • Forbidden sites:
    • SpeI (@228 T -> C)
    • PstI (@726 A -> T)
  • Undesirable sites:
    • None present in native sequence

Source

Natively found in [http://rebase.neb.com/rebase/enz/M.ScaI.html Streptomyces caespitosus].
[http://rebase.neb.com/cgi-bin/seqget?M.ScaI Native sequence] obtained from REBASE.
Synthesized by IDT as part of a XbaI+BBa_K874020+BBa_K874000+Scar+BBa_B0014+SpeI custom gene.[2]

References

  1. Knight, T. (2007). [http://dspace.mit.edu/handle/1721.1/45138 Draft Standard for Biobrick Biological Parts]. OpenWetWare
  2. IDT Gene Synthesis Specification Sheet for XbaI+BBa_K874020+BBa_K874000+Scar+BBa_B0014+SpeI Custom Gene