Primer

Part:BBa_K864446:Design

Designed by: Arvid Hedén Gynnå, Sabri Jamal, Lisa Branzell, Hampus Elofsson   Group: iGEM12_Uppsala_University   (2012-09-29)


Spot42 randomized reverse primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The melting temperature has been designed to match BBa_K864445.


Source

Own design.

References