Reporter
CBDclos(J6

Part:BBa_K863114:Design

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-23)

CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 1109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1020


Design Notes

This part is was made by a mixture of Freiburg-assembly of BBa_K863111 and BBa_K863121 and suffix insertion into BBa_J61101. Its purpose was to analyze of the expression problem of a similar construct with a T7-promoter.

Source

The source of the cellulose binding domain is a cloning-vector from the fermentations group of Bielefeld University. BBa_J61101 and GFP are from the parts.igem.

References