Coding

Part:BBa_K861070

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-18)

AdrA gene from E.coli K12

This is the coding sequence of gene AdrA, which contains a GGDEF domain that can catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activate bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. The gene can not be cloned using the standard provided by the registry. We therefore use the primer in the paper(Davide Antoniani et al. Appl Microbiol Biotechnol.2009) to clone this gene. This will lead to about 40bp genomic sequence added upstream the start codon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 523
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aAdrA gene from E.coli K12