Generator

Part:BBa_K861048

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-06)

PfadR regulated S-FadA and S-FadB

S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that they have far better efficiency compared to those in E.coli. We assembled S-FadA and S-FadB with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1539


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Categories
Parameters
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