Generator

Part:BBa_K861002

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-22)

Constitutively expressed FadL

This part was designed to characterize the effect of the gene FadL on fatty acid consumption. Long-chain fatty acids are transported across the cell membrane via a transportation/acyl-activation mechanism involving an outer membrane protein, FadL. The FadL gene encodes a 43,000-dalton membrane protein (FLP) which has been implicated as being essential for LCFA transportation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1166
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aConstitutively expressed FadL