Composite

Part:BBa_K855009:Design

Designed by: Ma Tsz Shan, Akansha Mahavir Shah   Group: iGEM12_HKU_HongKong   (2012-10-05)


AHL-inducible Production of pvdQ (K855006)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2284
    Illegal XhoI site found at 2682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1119
    Illegal NgoMIV site found at 1858
    Illegal NgoMIV site found at 2260
    Illegal NgoMIV site found at 2417
    Illegal NgoMIV site found at 2644
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005
    Illegal SapI.rc site found at 3014


Design Notes

Internal PstI site in pvdQ is removed by site-directed mutagenesis


Source

pseudomonas aeruginosa PAO1

References