Composite

Part:BBa_K855001:Design

Designed by: Akansha Mahavir Shah, Ma Tsz Shan   Group: iGEM12_HKU_HongKong   (2012-09-20)


AHL-inducible Production of pvdQ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2577
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2284
    Illegal XhoI site found at 2682
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2577
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2577
    Illegal NgoMIV site found at 1119
    Illegal NgoMIV site found at 1858
    Illegal NgoMIV site found at 2260
    Illegal NgoMIV site found at 2417
    Illegal NgoMIV site found at 2644
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005
    Illegal SapI.rc site found at 3014


Design Notes

The pvdQ gene contains an EcoRI-like site. This means that although the exact sequence of the EcoRI restriction enzyme is not present, EcoRI cuts the gene once. When digesting with EcoRI, a short incubation time is recommended to lessen the star activity.


Source

Pseudomonas aeruginosa PAO1

References