Part:BBa_K836003:Design

lipA from B. cepacia (codon usage optimized for E. coli)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 237
  Illegal BamHI site found at 1038
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 760
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence of a transcriptional terminator (biobrick BBa_B1006) was set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (https://parts.igem.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used (not in the sequence presented here).

Source

The amino sequence of this part was taken from Uniprot (lipA accesion P22088; http://www.uniprot.org/uniprot/P22088) and then codon optimization for E. coli (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.

References

1. Fjerbaek, L., Christensen, K. V., & Norddahl, B. (2009). A review of the current state of biodiesel production using enzymatic transesterification. Biotechnology and bioengineering, 102(5), 1298–315. doi:10.1002/bit.22256

2. Ghaly, A., Dave, D., Brooks, M., & Budge, S. (2010). Production of biodiesel by enzymatic transesterification: review. American Journal of Biochemistry and Biotechnology, 6(2), 54–76. Retrieved from http://www.cabdirect.org/abstracts/20103289908.html

3. Nelson, L. a., Foglia, T. a., & Marmer, W. N. (1996). Lipase-catalyzed production of biodiesel. Journal of the American Oil Chemists’ Society, 73(9), 1191–1195. doi:10.1007/BF02523383