Coding
DGAT

Part:BBa_K836002:Design

Designed by: Ricardo Alvarado   Group: iGEM12_Costa_Rica-TEC-UNA   (2012-09-18)


O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 40
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 418
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 192


Design Notes

Two transcriptional terminators (two biobricks BBa_B1006) were set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (https://parts.igem.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used.


Source

The amino sequence of this part was taken from Uniprot (wax-dgaT accesion Q8GGG1; http://www.uniprot.org/uniprot/Q8GGG1) and then codon optimization for R. opacus (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.

References