Coding

Part:BBa_K833012:Design

Designed by: Julianne Grose   Group: iGEM12_BYUProvo   (2012-10-02)


Lactate sensor derived from the MglB protein from E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 982
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 982
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 982
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designing primers to incorporate a large number of site-directed mutations


Source

This part was derived from site directed mutagenesis of the E. coli MglB protein.

References