Composite
Part:BBa_K824009:Experience
Designed by: Steven Allen Group: iGEM12_Gaston_Day_School (2012-10-02)
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Applications of BBa_K824009
User Reviews
UNIQa97689271a9b6120-partinfo-00000000-QINU UNIQa97689271a9b6120-partinfo-00000001-QINU
We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). We tested the detector with Cadmium since the promoter is responsive to multiple heavy metals. Due to safety issues, we were unable to use Arsenic because we are in BSL1 and it is a known poison. The results were measured in fluorescence per OD 600. The graph below shows our results.
