Plasmid
Sporovecto

Part:BBa_K823054:Design

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-09-23)


Sporovector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 766
    Illegal XbaI site found at 959
    Illegal PstI site found at 2543
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 766
    Illegal PstI site found at 2543
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 766
    Illegal BamHI site found at 748
    Illegal XhoI site found at 3538
    Illegal XhoI site found at 4456
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 766
    Illegal XbaI site found at 959
    Illegal PstI site found at 2543
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 766
    Illegal XbaI site found at 959
    Illegal PstI site found at 2543
    Illegal NgoMIV site found at 2035
    Illegal AgeI site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5383
    Illegal SapI.rc site found at 3132


Design Notes

For N-terminal fusion proteins with cgeA (display on B. subtilis spores)


Source

Assembly of several PCRs.

References