Coding

Part:BBa_K823020:Design

Designed by: Simon Kelterborn   Group: iGEM12_LMU-Munich   (2012-08-15)

chloramphenicol acetyltransferase (cat)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed the part in the freiburg standard (Assembly 25). We included a Bacillus optimized RBS between the XbaI and NgoIV restriction site. The part is delivered in pSB1A3 to avoid the same antibiotic resistance twice on a plasmid.

Source

Plasmid: Bacillus subtilis reporter vector pAC6

References