Signalling

Part:BBa_K819016:Design

Designed by: YU Zhou   Group: iGEM12_Peking   (2012-09-16)

Luminesensor repressible SulA408 promoter+CheZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CheZ coding sequence was placed under SulA408 promoter (BBa_K819017).

Source

CheZ was extracted from E.Coli strain MG1655 by colony PCR.
SulA408 promoter was from BBa_K819017

References

1.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of Escherichia coli. J. Bacteriol., 3:1307:1313
2.M. Germana Sanna and Melvin I. Simon(1996) In Vivo and In Vitro Characterization of Escherichia coli Protein CheZ Gain- and Loss-of-Function Mutants. J. Bacteriol., 178: 6275: 6280