Signalling
Part:BBa_K819015:Design
Designed by: YU Zhou Group: iGEM12_Peking (2012-09-16)
Luminesensor repressible RecA408 promoter+CheZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
CheZ coding sequence was placed under RecA408 promoter (BBa_K819002).
Source
CheZ was extracted from E.Coli strain MG1655 by colony PCR.
RecA408 promoter was from BBa_K819002.
References
1.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of Escherichia coli. J. Bacteriol., 3:1307:1313
2.M. Germana Sanna and Melvin I. Simon(1996) In Vivo and In Vitro Characterization of Escherichia coli Protein CheZ Gain- and Loss-of-Function Mutants. J. Bacteriol., 178: 6275: 6280