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Generator

Part:BBa_K819009:Design

Designed by: Xing Wenmin   Group: iGEM12_Peking   (2012-09-03)

Weak cheZ generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a point mutation at position 56 of CheZ coding sequence with have little effect to its function and the bases corresponding to stop codon is changed to bases coding a more frequently used stop codon.


Source

The promoter used here was from 2012 Spring Distribution Kit from Partsregistry. The CheZ gene was extracted from E.coli strain MG1655 by colony PCR and it is aligned with CheZ sequence at EcoGene database.

References

1.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of Escherichia coli. J. Bacteriol., 3:1307:1313
2.M. Germana Sanna and Melvin I. Simon(1996) In Vivo and In Vitro Characterization of Escherichia coli Protein CheZ Gain- and Loss-of-Function Mutants. J. Bacteriol., 178: 6275: 6280