Coding

Part:BBa_K818310

Designed by: Emeraldo Jo   Group: iGEM12_Groningen   (2012-09-24)

AmilGFP with alsT regulator

This is a coding construct for B. subtilis to produce AmilGFP pigment in response to rotten meat volatiles in the environment. AmilCP is a blue pigment designed by iGEM Uppsala 2011 and the regulator, alsT, is designed by iGEM Groningen 2012.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 465
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 465
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 465
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 465
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None