Regulatory

Part:BBa_K818200:Design

Designed by: Emeraldo Jo   Group: iGEM12_Groningen   (2012-09-19)

Regulator fnr, upregulated by rotten meat volatiles in B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.

Source

This part is from B. subtilis genome.

References

Reents H., Münch R., Dammeyer T., Jahn D., Härtig E. (2005).The Fnr Regulon of Bacillus subtilis. Journal of Bacteriology, 188(3):1103-1112