Part:BBa_K817034:Design

J23119-RBS-SP1-Penetratin-Flag-xx

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We add constitutive promoter part J23119 and RBS part B0030 in the front of K817027 and double terminator part B0015 for backward insertion.

For functional assay result, please refer to our [http://2012.igem.org/Team:NTU-Taida/Result/Penetration wiki page]

Source

J23119 - B0030 - K817027- B0015