Part:BBa_K817027:Design

SP1-Penetratin-Flag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We use Flag tag because Penetratin antibody is not available. We use Flag as label for Penetratin detection. Penetratin-Flag coding sequence is based on de novo synthesis amino acid sequence with E. coli codon optimization and signal peptide 1 is the signal peptide sequence of E.coli K12 strain PhoA protein.

Source

SP1 a.a. sequence: MKQSTIALALLPLLFTPVTKA
Penetratin a.a. sequence: RQIKIWFQNRRMKWKK
Flag a.a. sequence: DYKDDDDK