Coding

Part:BBa_K817000:Design

Designed by: Yen-Der Li   Group: iGEM12_NTU-Taida   (2012-09-24)

SP1-GLP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the coding sequence of Glucagon-Like Peptide-1 and our signal peptide 1. The latter will help to secret GLP-1 out of bacteria.

Source

GLP-1 coding sequence is from eukaryotic cells amino acid sequence with E. coli codon optimization and signal peptide 1 is the signal peptide sequence of E.coli K12 strain PhoA protein.
SP1 a.a. sequence: MKQSTIALALLPLLFTPVTKA
GLP-1 a.a. sequence: HAEGTFTSDVSSYLEGQAAKEFIAWLVKGRG

References