Device
Part:BBa_K812012:Design
Designed by: Cyrille Pauthenier Group: iGEM12_Evry (2012-09-18)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 433
Illegal NotI site found at 287 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1648
Illegal XhoI site found at 356 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 55
Illegal NgoMIV site found at 164 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 79
Design Notes
This part has been cloned in pSB1C3 using in-fusion and the illegal restriction site SpeI and PstI had been removed by PCR.
Source
This part has been extracted from the pNHK60 plasmid using PCR. THe pNHK60 plasmid was obtained from the riken registry.