Coding
Toc75_SPto

Part:BBa_K809326:Design

Designed by: Luohao Xu   Group: iGEM12_Shenzhen   (2012-09-26)


signal peptide of Tom40 + Toc75


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 629
    Illegal PstI site found at 1168
    Illegal PstI site found at 1687
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2473
    Illegal PstI site found at 629
    Illegal PstI site found at 1168
    Illegal PstI site found at 1687
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1911
    Illegal BamHI site found at 2338
    Illegal XhoI site found at 931
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 629
    Illegal PstI site found at 1168
    Illegal PstI site found at 1687
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 629
    Illegal PstI site found at 1168
    Illegal PstI site found at 1687
    Illegal NgoMIV site found at 1510
    Illegal NgoMIV site found at 2479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2047
    Illegal BsaI.rc site found at 1379
    Illegal SapI site found at 130
    Illegal SapI.rc site found at 890
    Illegal SapI.rc site found at 2119


Design Notes

Both parts should be linked correctly without nonsense mutation and frameshift mutation


Source

https://parts.igem.org/wiki/index.php?title=Part:BBa_K809209 https://parts.igem.org/wiki/index.php?title=Part:BBa_K809204

References