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Coding
LEV

Part:BBa_K807002:Design

Designed by: Ian Roney   Group: iGEM12_uOttawa_CA   (2012-09-28)


LEV: LexA DNA Binding Domain - Human Estrogen Receptor - VP16 Activating Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 112
    Illegal BglII site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 575
    Illegal BsaI.rc site found at 47


Design Notes

LexA DBD was yeast optimized and synthesized by IDT. We had to ensure that LexA DBD and hER did not have stop codons in order for the fusion protein to be built.


Source

LexA DBD - E.coli hER - Human VP16 - Herpes virus

References