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Coding

Part:BBa_K805013:Design

Designed by: Han Jiapeng   Group: iGEM12_HUST-China   (2012-09-20)

CcdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

sequence confirmed

Source

Extracted from the plasimd pEntr-3C

References

Hu LL, Zhang SS, Li XX, Wang BL.The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products.Mol Biol (Mosk). 2010 Jan-Feb;44(1):174-6.