Part:BBa_K804008:Experience

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UNIQ53b04a77f7e733c7-partinfo-00000000-QINU UNIQ53b04a77f7e733c7-partinfo-00000001-QINU

TrpA Knockout Complementation by British Columbia iGEM 2012

This part consists of an arabinose promoter, a strong RBS, and the TrpA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.

To generate this graph we cultured an E. coli TrpA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes. See BBa_K804009 for negative controls for this part as well as linked fluorescent data. Not understood