Generator

Part:BBa_K802000:Design

Designed by: Carine Gimbert, Anne Haziza   Group: iGEM12_Lyon-INSA   (2012-09-20)

Lysostaphin generator for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1316
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a strong RBS in order to produce high levels of expression of Lysostaphin.


Source

This is a composite part including the constitutive B. subtilis Pveg promotor and lysostaphin coding sequence.

References