Part:BBa_K801096:Design

Aromatic prenyltransferase (APT) coding region

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Synthesized by Geneart, yeast codon optimized

http://www.ncbi.nlm.nih.gov/nuccore/AB543053

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, naringenin-chalcone synthase, O-methyltransferase 1

Abbreviations: APT = aromatic prenyltransferase

Design Notes

Related BioBrick:

Related BioBricks:

• BBa_K801090: phenylalanine ammonia lyase (PAL) + yeast consensus sequence
• BBa_K801091: phenylalanine ammonia lyase (PAL) coding region
• BBa_K801092: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
• BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region
• BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence
• BBa_K801095: Naringenin - chalcone synthase (CHS) coding region
• BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence
• BBa_K801098: O-methyltransferase 1 (OMT1) coding region

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

• Protein: aromatic prenyltransferase
• Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: 2.5.1

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Synthesized by Geneart

Organism:
Genesequence derived from Humulus lupulus

Codonoptimized for Saccharomyces cerevisiae

References

Literature references:

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AB543053.1 GenBank: Humulus lupulus mRNA for aromatic prenyltransferase, complete cds]

[http://www.uniprot.org/uniprot/E5RP65 Uniprot: Aromatic prenyltransferase]