Coding

Part:BBa_K801072:Design

Designed by: Roman Prechtl   Group: iGEM12_TU_Munich   (2012-09-21)

3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 994
    Illegal BglII site found at 1090
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

http://www.ncbi.nlm.nih.gov/nuccore/AB084125

synthesized by IDT, yeast codon optimized
Keywords: koffein, caffeine, coffeine, coffee, xanthosine, coffea, arabica


Abbreviations: CaDXMT1 = coffea arabica 3,7-dimethylxanthine N-methyltransferase 1

Design Notes

Related BioBrick: BBa_K801075 BBa_K801077

Quality control measures:

  • Test digest has been performed with Xba1 and Pst1 (see mainpage)
  • Part was totally sequenced

Backbone:

  • pSB1C3

Protein coding:

  • CaDXMT1

Enzymatic activity: EC-number [http://enzyme.expasy.org/EC/2.1.1.160 2.1.1.160 ]

Cytotoxicity:
cytotoxic for S. cervisiae growth inhibition at 10 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • synthesized by IDT


Organism:

  • Genesequence derived from coffea arabica
  • codonoptimized for saccharomyces cerevisiae
  • designed for saccharomyces cerevisiae


References

Literature references:

  • [http://www.ncbi.nlm.nih.gov/pubmed/12746542 Pubmed: Uefuji H, Ogita S, Yamaguchi Y, Koizumi N, Sano H., 2003: Molecular cloning and functional characterization of three distinct n-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants. Plant Physiol]

Database references:

  • [http://www.ncbi.nlm.nih.gov/nuccore/AB084125 GenBank: Coffea arabica CaDXMT1 mRNA for 3,7-dimethylxanthine N-methyltransferase, complete cds]
  • [http://www.uniprot.org/uniprot/Q8H0D2 Uniprot: 3,7-dimethylxanthine N-methyltransferase]