Part:BBa_K801043:Design

LexA based yeast light-swithable promoter system

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 862
  Illegal BglII site found at 2795
  Illegal BglII site found at 5635
  Illegal BamHI site found at 2877
  Illegal XhoI site found at 2828
  Illegal XhoI site found at 2847
  Illegal XhoI site found at 4982
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2240
  Illegal AgeI site found at 5705
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4367
  Illegal BsaI site found at 4773
  Illegal BsaI.rc site found at 205
  Illegal BsaI.rc site found at 1986
  Illegal SapI site found at 3044
  

composite part of Bba_K319003, K801039, BBa_K801011 , Bba_K319003, Bba_K801041, and BBa_K801011

Keywords: PIF3, light switchable, LexA, GAL4

Abbreviations:

Design Notes

Related BioBrick:

• Related BioBricks:BBa_K319003: yeast TEF1 promoter, BBa_K801039: SV40NLS-PhyB-linker-PIF3, BBa_K801011: Part:BBa_K801011, BBa_K319003: yeast TEF1 promoter, BBa_K801041: SV40NLS-PhyB-linker-LexA, BBa_K801011: TEF1 yeast terminator

Cloning details:

• Designed in RFC25

Quality control measures:

Backbone:

• Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

References

Literature references:

Database references: