Regulatory

Part:BBa_K801010:Design

Designed by: Georg Sch�tzinger   Group: iGEM12_TU_Munich   (2012-09-21)

TEF2 constitutive yeast promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
  • 1000
    COMPATIBLE WITH RFC[1000]




Extracted from genomic DNA of S. cerevisiae using PCR.


Keywords:


Abbreviations:

Design Notes

Related BioBrick:BBa_K165037

Quality control measures:
Test digestion using XbaI+ PstI-HF, SpeI-HF+ PstI-HF

Backbone:
Backbone name: pSB1C3, Resistance: Cp

Protein coding:

Enzymatic activity:

Cytotoxicity:
none

Safety notes:
none

Intellectual property:

Corresponding part author/authors:

Source

Source:
PCR from genomic DNA of Saccharomyces cerevisiae

  • Forward Primer: VF2
  • Reverse Primer: VR

Organism:
Developed for Saccharomyces cerevisiae

References

Literature references:

  • Schirmaier, F.; Philippsen, P. (1984): Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae. In: EMBO J. 3 (13), S. 3311–3315.
  • Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.


Database references:

  • http://ypa.ee.ncku.edu.tw/index.html