Deleted
Sample Discontinued
Experience: None
Not Used
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Plasmid_Backbone

Part:BBa_K797011:Design

Designed by: Tomohiro Nobeyama   Group: iGEM12_Kyoto   (2012-09-21)


Plasmid include T7 promoter, Histag and R9 peptide sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1
    Illegal suffix found at 121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 122
    Illegal PstI site found at 136
    Illegal NotI site found at 7
    Illegal NotI site found at 129
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Illegal suffix found at 122
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal XbaI site found at 16
    Illegal SpeI site found at 122
    Illegal PstI site found at 136
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1126


Design Notes

Source

This part is synthesized artificially

References