Coding

Part:BBa_K797006:Design

Designed by: Tomohiro Nobeyama   Group: iGEM12_Kyoto   (2012-09-20)

FT protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 446
    Illegal BamHI site found at 210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 17


Design Notes

Wild type florigen colding region include intron and restriction enzyme cutting site of EcoR1 and Pst. We change its sequence and eliminate cutting site without changing amid acid sequence.


Source

This part is derived from Arabidopsis

References

[1]Araki, T et al.(2005) “FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex”. Science 309 (5737), 1052–1056