Part:BBa_K784019

Phage Lambda fragment 5 - recombination

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome.
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here].
Fragment 5 contains the recombination area that allows the phage lysogenic life cycle (penetration to the host genome). This part includes the att-λ sequence which allows site specific recombination into an E. coli genome (with the matching att-B sequence) between gal and bio operons.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4318
  Illegal PstI site found at 4576
  Illegal PstI site found at 4823
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4318
  Illegal PstI site found at 4576
  Illegal PstI site found at 4823
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4318
  Illegal BamHI site found at 543
  Illegal XhoI site found at 6069
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4318
  Illegal PstI site found at 4576
  Illegal PstI site found at 4823
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4318
  Illegal PstI site found at 4576
  Illegal PstI site found at 4823
  Illegal AgeI site found at 4396
  
• 1000
  COMPATIBLE WITH RFC[1000]