Composite
pT7_xyIE

Part:BBa_K784001:Design

Designed by: Noa Katz   Group: iGEM12_Technion   (2012-09-13)

pT7_RBS_xyIE reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 368
    Illegal NgoMIV site found at 540
    Illegal AgeI site found at 891
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure that the part doesn't have any xhoI or xmaI restriction sites in the gene.

Source

The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh. The promoter and terminator are from: http://nar.oxfordjournals.org/content/early/2012/06/28/nar.gks597.full


References