Composite
pT7+pHOA
Part:BBa_K784000:Design
Designed by: Lior Levy Group: iGEM12_Technion (2012-09-13)
pT7+RBS+Alkaline Phosphatase (pHO)+Terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1512
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1512
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1512
Illegal XhoI site found at 24 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1512
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1512
Illegal NgoMIV site found at 351
Illegal NgoMIV site found at 798 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
we used XhoI and XmaI restriction sites for the ligation because these sites are not anywhere in the sequence.
Source
The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.
The RBS is from the parts registry,BBa_B0064 made by Endi Lab (2008)
The pHOA gene is from the parts registry,BBa_J61032 made by Arkin Lab (2006)