Coding

Part:BBa_K783083:Design

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-03)


mmoR from M. trichosporium OB3b MoClo format


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1130
    Illegal PstI site found at 1406
    Illegal PstI site found at 2039
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1130
    Illegal PstI site found at 1406
    Illegal PstI site found at 2039
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 35
    Illegal XhoI site found at 407
    Illegal XhoI site found at 1883
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1130
    Illegal PstI site found at 1406
    Illegal PstI site found at 2039
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1130
    Illegal PstI site found at 1406
    Illegal PstI site found at 2039
    Illegal NgoMIV site found at 80
    Illegal NgoMIV site found at 262
    Illegal NgoMIV site found at 508
    Illegal NgoMIV site found at 776
    Illegal NgoMIV site found at 860
    Illegal NgoMIV site found at 1197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1103


Design Notes

The MoClo fusion sites were designed for this part.


Source

Methylosinus trichosporium OB3b

References