Plasmid

Part:BBa_K783061:Design

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-02)


Level 0 MoClo Destination Vector with DG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to confirm the lacZ orientation was 5'->3' after cloning.


Source

We amplified the alpha lacZ fragment from pUC19 and used the BioBrick pSB1C3 backbone for our vector backbone.

References