Plasmid

Part:BBa_K783055:Design

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-02)


Level 0 MoClo Destination Vector with AB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 251
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 251
    Illegal BamHI site found at 272
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 528


Design Notes

We had to confirm the lacZ orientation was 5'->3' after cloning.

Sequence given is flanked with SpeI sites as per the MoClo system.

Source

We amplified the alpha lacZ fragment from pUC19 and used the BioBrick pSB1C3 backbone for our vector backbone.

References