Part:BBa_K781006:Design

[R0010][B0034] - RFP-FliC Deletion Chimera

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1884
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 529
  Illegal AgeI site found at 1362
  Illegal AgeI site found at 1474
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera. Note that there are two different formats for making flagellin insertion variants and deletion variants

Source

This part was PCR amplified from J04450 and then overlap extension was used to incorporate it into the plasmid.