Composite

Part:BBa_K781004

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-03)

[R0010][B0034] - Rv2579 Dehalogenase-FliC Insertion Chimera

Rv2579 is a dehalogenase enzyme. This construct as incorporated that enzyme as an insertion into the variable domain.

Fig. 4 The motility assay for the K781004 construct compared against controls expressing no flagellin construct and a non-chimeric flagellin construct. Inoculations made on 0.25% swimming agar.






























Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2389
    Illegal XhoI site found at 1691
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1167
    Illegal NgoMIV site found at 1432
    Illegal AgeI site found at 530
    Illegal AgeI site found at 938
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None