Composite

Part:BBa_K781000:Design

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-01)


[R0010][B0034] - E. coli K12 FliC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 529
    Illegal AgeI site found at 937
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that when expressing this, and resulting overlaps in bacteria. One should be aware that this promoter is sensitive to LacI. Any strains overly expressing LacI will have very little expression of protein.


Source

The first constant domains and the D3 domain were amplified out of the separately, incorporating the appropriate silent mutations. The second constant domains were synthesized. The total coding domain was assembled using overlap extension PCR.

References