Generator

Part:BBa_K780004:Design

Designed by: iGEM 2012 Warsaw Team   Group: iGEM12_Warsaw   (2012-09-26)

RBS+GFP for expression in Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 666


Design Notes

-


Source

RBS was taken from 'host-takover module' from B.subtilis Bacteriophage SPO1 and GPF is derived from jellyfish Aequeora victoria (BBa_E0040).

References

1: Stewart CR, Gaslightwala I, Hinata K, Krolikowski KA, Needleman DS, Peng AS, Peterman MA, Tobias A, Wei P. Genes and regulatory sites of the "host-takeover module" in the terminal redundancy of Bacillus subtilis bacteriophage SPO1. Virology. 1998 Jul 5;246(2):329-40. PubMed PMID: 9657951.