Generator

Part:BBa_K779600

Designed by: Giulio Alighieri   Group: iGEM12_MIT   (2012-09-29)

Hef1a-eYFP MammoBlock Device a composite part of a Hef1A (human elongation factor) constitutive promoter with an engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces yellow that we created through the Gateway cloning protocol.
Testing eYFP driven by a Hef1A promoter (BBa_K779200)
EYFPchar.png
This image was taken on an Evos microscope at 10X. On the left, there is a brightfield image of 200,000 cells transiently transfected with Lipofectamine 2000 with 500 ng of Hef1A:eYFP. On the right, is an image taken in the FITC channel and we see high transfection efficiency and eYFP expression 48 hours post-transfection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1407
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 569
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1407
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1407
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHef1a-eYFP MammoBlock Device